Composite

Part:BBa_K909014:Design

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-22)

Constitutive expression of pABA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Built from two existing biobricks: BBa_K137055 (promoter removed by PCR) and BBa_S04039.

Source

see corresponding Biobricks.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855612/ Wegkamp et al. (2007) "Characterization of the Role of para-Aminobenzoic Acid Biosynthesis in Folate Production by Lactococcus lactis"]

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177419/ V K Viswanathan, J M Green and B P Nichols. (1995) "Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli."]